HybSelect™ Service for next generation sequencing
Overview of Method
Recent technical progress has increased the throughput of DNA sequencing dramatically. However, this increase in throughput has had the consequence of exceeding the capacity of today's sample preparation techniques. The main limitation to faster and efficient sequence data mining is the complexity of higher organisms' genomes with the corresponding problem of large scale targeting of interesting DNA sequences. Methods to overcome this limitation, such as PCR amplification with specific primer pairs, or screening of bacterial libraries, are time-consuming and cost-intensive. The solution to this problem is the fast and flexible generation of a huge number of specified oligonucleotides for use as capture probes to enrich defined DNA sequences. Preselection of individual DNA fragments out of a complex background of genomic DNA can be accomplished using customized oligonucleotide microarrays synthesized by febit’s Geniom® platform.
HybSelect™ from febit is a method for the specific reduction of complexity in samples of genomic DNA, termed genome partitioning. The final product of genome partitioning is a DNA sample that is enriched in sequences that are of most interest to the researcher. This method can be used in combination with all NGS devices, including Applied Biosystems’ SOLiD, Solexa’s Illumina sequencer and Roche's 454 instruments.
Using HybSelect™ saves time, costs and technical resources by carrying out a targeted sequencing on those regions of genomic DNA that are of interest to you. HybSelect™ opens new gates for high throughput comparisons of individual samples, e.g. comparison of humans genomes - finding of disease related changes.
How does it work?
Here is an overview of the HybSelect™ method for genome partitioning
During the sequencing protocol for NGS platforms, the sample is fragmented and adaptors are ligated onto the fragments to prepare a DNA library. This library can now be hybridized to one, or multiple arrays of a Geniom® Biochip. Each array is synthesized to include capture probes that are specific for the genomic DNA target of interest. The extremely high flexibility of Geniom Biochips means that every array can be customized to select any target sequence of interest.
During the HybSelect™ genome partitioning process, we include appropriate controls to ensure:
- best biochip quality
- best enrichment is occurring.
The HybSelect™ method only requires 2 µg of sample. This sample is hybridized to the array overnight, under active motion hybridization, enabled by the advanced microfluidics of the Geniom® platform. The array is then washed in order to remove any residual, non-target DNA. Target genomic DNA will later be recovered from the array and is now ready for incorporation into the protocol of an NGS instrument.
Are you interested to learn more?
febit plans to launch HybSelect™ as a service in 2008. Customers will be able to send DNA samples to us, specifying regions of interest, and we will ship enriched DNA back to you. The DNA will be suitable for all NGS instrument platforms. Later, febit will launch HybSelect™ as an application that can be used directly in the laboratory.
Europe and other countries
febit biomed
Phone: +49 (0)6221 – 6510-300
Fax: +49 (0)6221 – 6510-329
E-mail: info@febit.eu
Americas
febit inc.
Phone: +1 781-391-4360
Toll free: 877-Geniom1 (or 877-436-4661)*
Fax: +1 781-391-4362
E-mail: info@febit.com