Dynamic detection
The Geniom platform allows specific measurements and quality control via real-time monitoring on the chip: the dynamic detection of hybridization and amplification!
Presenting this novel measuring principle, Geniom enables advanced applications and new approaches.
Dynamic detect ion: High-resolution in terms of quality and intervals of digital imaging
Dynamic detection, the generation of CCD-images at defined time points, as a standard feature of the Geniom technology is a key aspect of quality control. Digital imaging within the scope of a specific temperature program allows for precise monitoring of every single reaction on a microarray, the determination of melting points as well as the quantification of double stranded DNA during the annealing phase.
Dynamic detection features:
- Generation of melting curves to ensure product specificity, particularly for enzyme-based applications on microarrays. The selective and sensitive detection of the fluorophores gives the user full control of his experiments.
- High-resolution in terms of quality and intervals of the acquired pictures allows user-defined, synchronized checks of every individual reaction in the microchannels of the Geniom biochip.
- Dynamic detection represents an ideal selection tool for the determination and optimization of the right and specific probes, since probe-sample behaviour is not just predicted, but can be checked at any temperature during the experiment.
- Documentation and analysis of the dynamic hybridization enables a specific selection process to ensure ideal probe composition for every analyzed sequence.
- For the generation of genomic data many microarray platforms often sacrifice specificity for scale, yielding very large quantities of relatively lower-quality data than traditional experimental methods. With the dynamic detection as a standard feature, the Geniom technology eliminates questionable results.
- Employing the convenient Geniom software, the system automatically keeps track of every single reaction, integrating quality control checks to ensure reliable data and integrity of results.
Advanced applications through real-time monitoring
System features, including the microfluidic system and a temperature range from room temperature to 80 °C, enable user-specified experimental procedures and customized monitoring of any spot on the microarray.
The Geniom instrument integrates design, production and analysis of dedicated DNA biochips for all kinds of user-determined microarray applications:
- convenient and quick analyses of predesigned ready-to-go arrays
- applications like SNP typing or Dynamic Allele-Specific Hybridization (DASH) are supported by this additional feature, since synchronized monitoring during the heating process allows high-level sequence determination at a single base resolution.
- use of febit’s 3’- inversed synthesis set for reverse (3’-up) synthesis of DNA oligonucleotides in the biochip. The emerging probes present free 3’-ends accessible to enzymes like nucleic acid polymerases, providing a broad spectrum of new enzyme based applications for DNA microarrays, like PCR-on-chip or primer extension reactions.
These applications benefit from the dynamic detection, since the technology offers real-time control over temperature and fluorescence monitoring to check for amplification rate and product specificity.
With its multitude of applications, customisable set up, high throughput analysis of virtually any sequence data from any species, the Geniom is a powerful platform to face the many and varying challenges experienced by today’s genomic researchers. Geniom’s dynamic detection adds reassurance and quality to the accuracy and specificity of microarray performance, providing you with accurate reliable results.