microRNA Profiling
microRNAs (miRNAs) are a class of evolutionary conserved, approximately 22-nucleotide non-coding RNAs that have recently emerged as important regulators of gene expression. They are involved in the regulation of many key biological processes by influencing the translational status of the transcriptome.
A direct involvement in human diseases, such as cancer and diabetes, has been implied and highlights the need to obtain detailed expression profiles of miRNAs in tissues, during development, and in disease. The unexpected abundance and functional significance of noncoding RNAs, however, challenges the adaptation of current technologies to enable accurate miRNA profiling.
Their small size and the existence of miRNA families of related sequences pose critical problems in evaluating miRNA expression, especially using high-throughput approaches. Also, keeping the pace with fast changing database information and newly discovered miRNAs is difficult to manage with current approaches like ready-made microarrays.
febit’s Geniom technology addresses the special requirements for the analysis of miRNA expression, with its biggest advantage being the extraordinary adaptability to up-to-date information. Keeping the pace with ever-changing updates of the miRNA databases as well as easy to handle adjustments of microarray setups enable modern analyses of miRNA profiles.
Geniom features for miRNA profiling:
- platform enables fast and reliable analyses of miRNA profiles
- high-throughput analysis of small RNA expression, including general miRNA profiling
- comparative studies of disease versus normal tissue together on the same array
- identification of novel miRNAs
- detect mature miRNAs or precursor molecules
- quick adjustment of microarray setups / newest sequence information from miRNA databases can easily be integrated into Geniom ready to go miRNA microarrays
- ready to go miRNA-arrays for quick and easy analysis with the Geniom Analyzer
