Feature Recognition
Data Detection
- let automated feature recognition replace tedious manual spot-finding
After hybridization, re-insertion of the Geniom biochip into the Geniom biochip slot, and filter selection, Geniom will automatically scan the arrays and detect the fluorescence emitted from each feature. febit’s software will perform background subtraction, normalization and noise reduction. Data are stored in the database.
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1. Selecting a GeniomChoose the Geniom instrument you wish to use for detection.
On the left side of the screen, select one of the available Geniom instruments and drag it to the right side of the screen or click „add“.
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2. Choosing a Biochip for DetectionThe Geniom client keeps track of all your Biochips including those who have already been used in a previous experiment. For each individual Biochip, it stores the syntheses, hybridizations and detections that have been performed on it.
On the left side of the screen, select the appropriate Biochip and drag it to the right side of the screen or click „add“.
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3. Starting the DetectionConfigure detection parameters and start image acquisition.
The resulting image will be displayed. Proceed to feature extraction.
Current detection parameter settings are listed on the right side of the screen.
These include:
- Auto exposure time
- Mean over 16 images for enhanced signal-to-noise ratio (yes/no)
- Hardware gain for weak signals (yes/no)
- Excitation and detection filters (Cy3, Cy5, FRET or FITC).
- Background subtraction (yes/no)
Thumbnails of all detection images acquired for the Biochip are displayed. Double-click for a full-scale view.
