Detection
During detection, incident light guided via fiber optics exposes the biochip in a dark field.
A range of optical filters ensures a high-yield of appropriate wavelengths for selective excitation of fluorescence dyes. Filters are changed via Geniom software.
Geniom detects fluorescence-labeled target molecules bound to the immobilized oligonucleotides in the biochip via digital imaging.
Its built-in 8 megapixel CCD camera uses a high-aperture macro lens and records an image of the fluorescence pattern on the biochip surface. A background image is subtracted from the actual sample recording. The image is saved as a standard tiff file.
Geniom calculates signal intensities from the image data and allocates them to the corresponding features. Results are saved as standard txt files and may be exported as such or as Excel data.
Since Geniom uses a low-bleaching detection method, you can wash and detect your array several times and store pictures and raw data until you reach optimum results genes with high and low level expression.
Since all data are stored in the Geniom database, each result ever obtained with each probe is readily accessible and genes may be easily traced throughout the workflow.
Detection the easy way
- Insert the hybridized biochip into the Geniom processor slot
- Select the optical filter appropriate for the label of your target sample
- Geniom will then automatically
- scan the arrays and detect the fluorescence emitted from each array feature
- perform background subtraction, normalization and noise reduction
Different types of data analysis routines are available:
- Expression profiling routines calculate expression levels and fold changes
- Genotyping routines enable SNP calling and differentiation of allele zygosity Raw data and results may be exported in standard formats (txt, Excel) for further use in various contexts.