DNA-Synthesis
Spots of light: on-site synthesis of real-time microarrays
- principle: maskless light-activated in situ oligonucleotide synthesis
- light source: high-pressure mercury discharge lamp (350-750 nm)
- light projection: digital deflectable micromirror device
- (DMD, produced by Texas Instruments) of 786,432 individually addressable mirrors
Geniom automatics: automated array synthesis
Geniom automatically
- maps the array design on the biochip
- links the photolabile-protected spacer to the glass surface
- deprotects the spacer in selected spots by the micromirror-directed light beam
- adds protected amidite DNA building blocks that will couple only on deprotected spots
- builds up specific DNA-sequences by repeated cycles of selective light-activated deprotection and coupling of amidites
- enables sample hybridization by a final activation step
Geniom flexibility: tailored oligonucleotide synthesis
- any oligonucleotide sequence
- any length (10-60mers) - even within one array (compensate for different hybridization temperatures of probes)
- any probe parameter setting (optimized AT/GC content or melting point)
- any array design accessible within hours
Novel chemistries: novel applications
- Geniom enables 5´-3´synthesis enzymatic reactions, like in situ primer extension experiments inside the biochip
- Spacers bound to activated glass surface
- Selective cleavage of protection groups at illuminated spots
- Coupling of amidite building blocks results in oligonucleotide chain elongation
- Synthesis of up to 60mers runs overnight. Depending on your hybridization protocol you have the results ready 24 hours after starting the array design.
Maskless, light-activated oligonucleotide synthesis
A digital micromirror device (DMD) carries out light-activated, spatially resolved deprotection of photolabile groups immobilized inside the Geniom biochip microchannels. Deflection of single micromirrors is addressed by software control. When amidite DNA building blocks are added, coupling takes place exclusively at those spots which have been deprotected. Repeated cycles of photodeprotection and amidite coupling result in oligonucleotides with sequences specific for each spot. The current Geniom synthesis sets support 3’ to 5’ and 5’ to 3’ synthesis of 10- to 30mer oligonucleotides. Longmer sets for standard synthesis of up to 60mers are also available from febit